FBIP: Survey of invertebrate and microbial diversity of the Prince Edward Islands system

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如何引用

研究者應依照以下指示引用此資源。:

Dorrington R (2021): FBIP: Survey of invertebrate and microbial diversity of the Prince Edward Islands system. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=fbip111&v=1.0

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 South African National Biodiversity Institute。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 5c23cc27-be23-48fe-bd70-a0cef232138d。  South African National Biodiversity Institute 發佈此資源，並經由South African Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。

關鍵字

Occurrence; Sagina procumbens; Azorella selago; plant rhizosphere; soil microbial communities; Penicillium; Trichoderma; Ascomycetes; Specimen; Prince Edward Islands (Marion and Prince Edward); polar ecosystems; climate change; biodiversity survey; Invertebrates; microbials; terrestrial habitats

聯絡資訊

地理涵蓋範圍

Prince Edward and Marion Islands

分類群涵蓋範圍

microbial diversity

時間涵蓋範圍

計畫資料

Environmental metagenomic data generated from sequencing amplicon of in-shore, off-shore and plant-associated soil microbial communities of the sub-Antarctic Prince Edward and Marion Islands

參與計畫的人員:

取樣方法

- Soil/root samples were collected from various plants occuring on Marion Island, these samples were stored at -20oC and sent to the laboratory for analysis. Despite being frozen isolations were performed. - Surface seawater (taken from a depth of 5 m) and for the illumina samples (depth was approx 5m) was filtered through 100 µm mesh, followed by polyethersulfone membrane filters (0.22 µm) to collect bacteria. Filtered seawater and the filters were stored separately at -20ºC.

方法步驟描述:

1. Standard mycological culturing methods, used standard sequencing methods: - 16S rRNA genes using bacterial targeting and archaea targeting primers and 18S rRNA genes using universal eukaryote primers were amplified via polymerase chain reactions using genomic DNA extracted from biomass which was collected on the polyethersulfone membrane filters. Polymerase chain reaction products were sized on an agarose gel and extracted. The cleaned up extractions were then sequenced via pyrosequencing and Illumina sequencing. Sequence reads were curated using Mothur. - Extraction of genomic DNA from soil samples, followed by polymerase chain reaction (PCR) amplification of 16S rRNA and 18S rRNA gene for selected samples. PCR products were cut out from agarose gels to ensure the correct PCR product was obtain, each correct product was extracted from the gel and then purified, followed by sequencing on the Illumina platform. Raw sequence reads were curated using Mothur and classified according to the Silva v132 database. - Extraction of genomic DNA from root samples, followed by polymerase chain reaction (PCR) amplification of the ITS1 region. PCR products were cut out from agarose gels to ensure the correct PCR product was obtain, each correct product was extracted from the gel and then purified, followed by sequencing on the Illumina platform. - DNA was extracted from the isolated fungal endophytes, the ITS region was PCR amplified and amplicons were sent for sanger sequencing. When sequences were determined individual isolates were identified using BLAST on the NCBI website

額外的詮釋資料