FBIP: Survey of invertebrate and microbial diversity of the Prince Edward Islands system

Ocorrência
Versão mais recente published by South African National Biodiversity Institute on mar 31, 2021 South African National Biodiversity Institute
Publication date:
31 de março de 2021
Licença:
CC-BY 4.0

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Descrição

Environmental metagenomic data generated from sequencing amplicon of in-shore, off-shore and plant-associated soil microbial communities of the sub-Antarctic Prince Edward and Marion Islands

Registros de Dados

Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 14.021 registros.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Dorrington R (2021): FBIP: Survey of invertebrate and microbial diversity of the Prince Edward Islands system. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=fbip111&v=1.0

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é South African National Biodiversity Institute. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 5c23cc27-be23-48fe-bd70-a0cef232138d.  South African National Biodiversity Institute publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por South African Biodiversity Information Facility.

Palavras-chave

Occurrence; Sagina procumbens; Azorella selago; plant rhizosphere; soil microbial communities; Penicillium; Trichoderma; Ascomycetes; Specimen; Prince Edward Islands (Marion and Prince Edward); polar ecosystems; climate change; biodiversity survey; Invertebrates; microbials; terrestrial habitats

Contatos

Rosemary Dorrington
  • Provedor Dos Metadados
  • Originador
  • Ponto De Contato
Professor
Rhodes University
Room 515, Biological Sciences Building, Prince Alfred Road
6140 Grahamstown
Eastern Cape
ZA
+27 46 6038442
Mahlatse Kgatla
  • Distribuidor
Data Quality Specialist
SANBI
2 Cussonia Avenue
0184 Pretoria
Gauteng
ZA
0128435196

Cobertura Geográfica

Prince Edward and Marion Islands

Coordenadas delimitadoras Sul Oeste [-47,5, 37,3], Norte Leste [-46,5, 38,5]

Cobertura Taxonômica

microbial diversity

Reino Bacteria, Archaea, Eukaryota, Fungi

Cobertura Temporal

Data Inicial / Data final 2012-04-25 / 2016-05-28

Dados Sobre o Projeto

Environmental metagenomic data generated from sequencing amplicon of in-shore, off-shore and plant-associated soil microbial communities of the sub-Antarctic Prince Edward and Marion Islands

Título FBIP: Survey of invertebrate and microbial diversity of the Prince Edward Islands system
Identificador FBIS150518118100
Financiamento Foundational Biodiversity Information Programme
Descrição da Área de Estudo Prince Edward and Marion Islands

O pessoal envolvido no projeto:

Rosemary Dorrington
  • Proprietário

Métodos de Amostragem

- Soil/root samples were collected from various plants occuring on Marion Island, these samples were stored at -20oC and sent to the laboratory for analysis. Despite being frozen isolations were performed. - Surface seawater (taken from a depth of 5 m) and for the illumina samples (depth was approx 5m) was filtered through 100 µm mesh, followed by polyethersulfone membrane filters (0.22 µm) to collect bacteria. Filtered seawater and the filters were stored separately at -20ºC.

Área de Estudo Survey and metabarcoding of microorganism in Prince Edward and Marion Islands
Controle de Qualidade No

Descrição dos passos do método:

  1. Standard mycological culturing methods, used standard sequencing methods: - 16S rRNA genes using bacterial targeting and archaea targeting primers and 18S rRNA genes using universal eukaryote primers were amplified via polymerase chain reactions using genomic DNA extracted from biomass which was collected on the polyethersulfone membrane filters. Polymerase chain reaction products were sized on an agarose gel and extracted. The cleaned up extractions were then sequenced via pyrosequencing and Illumina sequencing. Sequence reads were curated using Mothur. - Extraction of genomic DNA from soil samples, followed by polymerase chain reaction (PCR) amplification of 16S rRNA and 18S rRNA gene for selected samples. PCR products were cut out from agarose gels to ensure the correct PCR product was obtain, each correct product was extracted from the gel and then purified, followed by sequencing on the Illumina platform. Raw sequence reads were curated using Mothur and classified according to the Silva v132 database. - Extraction of genomic DNA from root samples, followed by polymerase chain reaction (PCR) amplification of the ITS1 region. PCR products were cut out from agarose gels to ensure the correct PCR product was obtain, each correct product was extracted from the gel and then purified, followed by sequencing on the Illumina platform. - DNA was extracted from the isolated fungal endophytes, the ITS region was PCR amplified and amplicons were sent for sanger sequencing. When sequences were determined individual isolates were identified using BLAST on the NCBI website

Metadados Adicionais