說明
Isolated cultures and environmental metagenomic sequencing data using a target DNA markers ITS region for Fungi
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版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Dames J (2020): FBIP: Mycorrhizal and root endophytic fungi associated with Orchids. v1.1. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=dames_ru_2017_v2&v=1.1
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 South African National Biodiversity Institute。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: f703b08e-008e-438c-ad2d-58d544825692。 South African National Biodiversity Institute 發佈此資源，並經由South African Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence; Specimen
聯絡資訊
- 元數據提供者 ●
- 出處 ●
- 連絡人
- 散布者
地理涵蓋範圍
South Africa (Eastern Cape, KwaZulu-Natal)
| 界定座標範圍 | 緯度南界 經度西界 [-33.334, 26.493], 緯度北界 經度東界 [-28.234, 31.191] |
|---|
分類群涵蓋範圍
The isolates and metagenomic sequences are mycorrhizal and other root endophytic fungi that are found within orchid roots
| Phylum | Fungi |
|---|
時間涵蓋範圍
| 起始日期 / 結束日期 | 2017-05-01 / 2018-11-29 |
|---|
計畫資料
Isolated cultures and environmental metagenomic sequencing data using a target DNA markers ITS region for Fungi
| 計畫名稱 | FBIP: Mycorrhizal and root endophytic fungi associated with Orchids |
|---|---|
| 辨識碼 | FBIS170410226411 |
| 經費來源 | Foundational Biodiversity Information Programme |
| 研究區域描述 | South Africa (Eastern Cape, KwaZulu-Natal) |
參與計畫的人員:
- 研究主持人
取樣方法
1 g of orchid root material was collected from each orchid species, replicated 6 times, roots were surface sterilized and were etiher stored in RNA Later and frozen for molecular analysis or they were were sliced into smaller segments and placed onto suitable media to isolate endophytic fungi.
| 研究範圍 | South Africa (Eastern Cape, KwaZulu-Natal) |
|---|---|
| 品質控管 | The coordinates of the location where samples were collected was recorded |
方法步驟描述:
- Fungal isolates: Sampled root sections were surface sterilized and plated onto Malt Extract Agar. After incubation sub-cultures were made to ensure pure cultures were maintained. DNA was extracted from the cultures and the ITS region was amplified using ITS1 and ITS 4 primers. After purification amplicons were sent for Sanger Sequencing and submitted to GenBank for identification. Root samples: Genomic DNA was extracted from root samples, followed by polymerase chain reaction (PCR) amplification of the ITS region using Miseq tagged primers. Amplicon were submitted to SAIAB for Illumina Next Generation Sequencing.Data was analysed using Mothur software.