Descrição
Isolated cultures and environmental metagenomic sequencing data using a target DNA markers ITS region for Fungi
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 1.197 registros.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Dames J (2020): FBIP: Mycorrhizal and root endophytic fungi associated with Orchids. v1.1. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=dames_ru_2017_v2&v=1.1
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é South African National Biodiversity Institute. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: f703b08e-008e-438c-ad2d-58d544825692. South African National Biodiversity Institute publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por South African Biodiversity Information Facility.
Palavras-chave
Occurrence; Specimen
Contatos
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
- Distribuidor
Cobertura Geográfica
South Africa (Eastern Cape, KwaZulu-Natal)
| Coordenadas delimitadoras | Sul Oeste [-33,334, 26,493], Norte Leste [-28,234, 31,191] |
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Cobertura Taxonômica
The isolates and metagenomic sequences are mycorrhizal and other root endophytic fungi that are found within orchid roots
| Filo | Fungi |
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Cobertura Temporal
| Data Inicial / Data final | 2017-05-01 / 2018-11-29 |
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Dados Sobre o Projeto
Isolated cultures and environmental metagenomic sequencing data using a target DNA markers ITS region for Fungi
| Título | FBIP: Mycorrhizal and root endophytic fungi associated with Orchids |
|---|---|
| Identificador | FBIS170410226411 |
| Financiamento | Foundational Biodiversity Information Programme |
| Descrição da Área de Estudo | South Africa (Eastern Cape, KwaZulu-Natal) |
O pessoal envolvido no projeto:
- Pesquisador Principal
Métodos de Amostragem
1 g of orchid root material was collected from each orchid species, replicated 6 times, roots were surface sterilized and were etiher stored in RNA Later and frozen for molecular analysis or they were were sliced into smaller segments and placed onto suitable media to isolate endophytic fungi.
| Área de Estudo | South Africa (Eastern Cape, KwaZulu-Natal) |
|---|---|
| Controle de Qualidade | The coordinates of the location where samples were collected was recorded |
Descrição dos passos do método:
- Fungal isolates: Sampled root sections were surface sterilized and plated onto Malt Extract Agar. After incubation sub-cultures were made to ensure pure cultures were maintained. DNA was extracted from the cultures and the ITS region was amplified using ITS1 and ITS 4 primers. After purification amplicons were sent for Sanger Sequencing and submitted to GenBank for identification. Root samples: Genomic DNA was extracted from root samples, followed by polymerase chain reaction (PCR) amplification of the ITS region using Miseq tagged primers. Amplicon were submitted to SAIAB for Illumina Next Generation Sequencing.Data was analysed using Mothur software.
Metadados Adicionais
| Identificadores alternativos | http://ipt.sanbi.org.za/iptsanbi/resource?r=dames_ru_2017_v2 |
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