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如何引用
研究者應依照以下指示引用此資源。:
Pietersen G (2020): FBIP: Taxonomy and Candidatus Liberibacter status of South African Buchu plants. v1.1. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=taxonomy&v=1.1
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此資料的發布者及權利單位為 South African National Biodiversity Institute。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 065c4d4a-eead-44a4-ac94-19df2a0a47ad。 South African National Biodiversity Institute 發佈此資源，並經由South African Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence; Specimen
聯絡資訊
資源建立者:
可回覆此資源相關問題者:
元數據填寫者:
與此資源的相關者:
地理涵蓋範圍
South Africa (Western Cape, Kwa-Zulu Natal)
| 界定座標範圍 | 緯度南界 經度西界 [-34.778, 17.71], 緯度北界 經度東界 [-26.746, 33.223] |
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分類群涵蓋範圍
Family: Rutaceae
| Family | Rutaceae (Buchus Plants) |
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時間涵蓋範圍
| 起始日期 / 結束日期 | 2014-10-16 / 2018-01-12 |
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計畫資料
Survey of Rutaceae species of the fynbos area. Additional data, will help provide abundance lists, distribution and locality of these plants, while the molecular means to identify the species.
| 計畫名稱 | Taxonomy and Candidatus Liberibacter status of South African Buchu plants |
|---|---|
| 辨識碼 | FBIS150609119002 |
| 經費來源 | Foundational Biodiversity Information Programme |
| 研究區域描述 | South Africa (Western Cape, Kwa-Zulu Natal) |
參與計畫的人員:
取樣方法
Leaves were collected
| 研究範圍 | South Africa (Western Cape, Kwa-Zulu Natal) |
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方法步驟描述:
- 1) We plan to collect and sequence barcoding genes of known, morphologically identified specimens of buchu collected under permit at Kirstenbosch botanical gardens or from herbarium specimens where permission is granted to do so. Collection from live plants will not be destructive as only about 5g fresh weight of leaf material is required in order to obtain phloem tissue (to which the Ca. Liberibacters are confined). 2) We will collect leaf material from large numbers of buchu specimens (we plan to collect a total of about 1000 specimens in total, with between 10 and 20 replicates of visually identified species of “buchu” (in the broadest sense) from randomly selected transects from three Western Cape nature reserves containing the greatest diversity of buchu, and where permits for the study can be obtained. This will be done in the flowering season of two successive years. All samples obtained will be assigned a unique accession number and the following data will be recorded for each specimen: date of collection, collector details, photographs of the plant and any distinguishing feature(s), plant visual identification, locality data, the exact location (to an accuracy of 0.25m determined by a differential GPS) and the habitat description. 3) Collected material will be archived in the South African Plant Virus culture collection (SAPVC) at ARC-PPRI, as desiccated plant material as well as in -80oC freezer storage, and voucher specimens for identification purposes submitted to the Compton Herbarium (NBG) and National Herbarium (PRE). 5) All samples will be tested for Ca. Liberibacter using a generic Ca. Liberibacter PCR developed in our laboratory (Pietersen, unpublished), based on all known Ca. Liberibacter sequences. 6) All Liberibacter infected samples (as determined by real-time PCR) will be tested with primers to specific Liberibacters (Ca. Liberibacter -americanus, -africanus, - asiaticus, -solanacearum and -africanus subspecies –capensis, -verpidis, -clausenae, -zanthoxyli, and –tecleae) in conventional PCR’s or a newly developed multiplex PCR capable of detecting these (Roberts unpublished results). 7) All plant samples, irrespective of Ca. Liberibacter status, will be subjected to barcoding PCR’s (rbcL, matK, trnH/psb) and the amplicons sequenced by Sanger sequencing.