FBIP: Taxonomy and Candidatus Liberibacter status of South African Buchu plants

Registros de Dados

Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 834 registros.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Pietersen G (2020): FBIP: Taxonomy and Candidatus Liberibacter status of South African Buchu plants. v1.1. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=taxonomy&v=1.1

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é South African National Biodiversity Institute. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 065c4d4a-eead-44a4-ac94-19df2a0a47ad.  South African National Biodiversity Institute publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por South African Biodiversity Information Facility.

Palavras-chave

Occurrence; Specimen

Contatos

Cobertura Geográfica

South Africa (Western Cape, Kwa-Zulu Natal)

Cobertura Taxonômica

Family: Rutaceae

Cobertura Temporal

Dados Sobre o Projeto

Survey of Rutaceae species of the fynbos area. Additional data, will help provide abundance lists, distribution and locality of these plants, while the molecular means to identify the species.

O pessoal envolvido no projeto:

Métodos de Amostragem

Leaves were collected

Descrição dos passos do método:

1. 1) We plan to collect and sequence barcoding genes of known, morphologically identified specimens of buchu collected under permit at Kirstenbosch botanical gardens or from herbarium specimens where permission is granted to do so. Collection from live plants will not be destructive as only about 5g fresh weight of leaf material is required in order to obtain phloem tissue (to which the Ca. Liberibacters are confined). 2) We will collect leaf material from large numbers of buchu specimens (we plan to collect a total of about 1000 specimens in total, with between 10 and 20 replicates of visually identified species of “buchu” (in the broadest sense) from randomly selected transects from three Western Cape nature reserves containing the greatest diversity of buchu, and where permits for the study can be obtained. This will be done in the flowering season of two successive years. All samples obtained will be assigned a unique accession number and the following data will be recorded for each specimen: date of collection, collector details, photographs of the plant and any distinguishing feature(s), plant visual identification, locality data, the exact location (to an accuracy of 0.25m determined by a differential GPS) and the habitat description. 3) Collected material will be archived in the South African Plant Virus culture collection (SAPVC) at ARC-PPRI, as desiccated plant material as well as in -80oC freezer storage, and voucher specimens for identification purposes submitted to the Compton Herbarium (NBG) and National Herbarium (PRE). 5) All samples will be tested for Ca. Liberibacter using a generic Ca. Liberibacter PCR developed in our laboratory (Pietersen, unpublished), based on all known Ca. Liberibacter sequences. 6) All Liberibacter infected samples (as determined by real-time PCR) will be tested with primers to specific Liberibacters (Ca. Liberibacter -americanus, -africanus, - asiaticus, -solanacearum and -africanus subspecies –capensis, -verpidis, -clausenae, -zanthoxyli, and –tecleae) in conventional PCR’s or a newly developed multiplex PCR capable of detecting these (Roberts unpublished results). 7) All plant samples, irrespective of Ca. Liberibacter status, will be subjected to barcoding PCR’s (rbcL, matK, trnH/psb) and the amplicons sequenced by Sanger sequencing.

Metadados Adicionais