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Researchers should cite this work as follows:
Oosthuizen M (2020): FBIP: Cataloguing zoonotic tick-borne bacterial pathogen strain diversity in wild rodent species in rural South Africa. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=fbip2&v=1.0
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The publisher and rights holder of this work is South African National Biodiversity Institute. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
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South Africa (Mpumalanga)
|Bounding Coordinates||South West [-24.827, 31.33], North East [-24.617, 32.371]|
Catalogue the tick-borne bacterial pathogen strain diversity present in wild rodent species
|Genus||Anaplasma (Bacteria), Mastomys (Rodents)|
|Start Date / End Date||2012-04-01 / 2016-04-05|
The aim of the study is, therefore, to catalogue the tick-borne bacterial pathogen strain diversity present in wild rodent species in the Mnisi community that could impact human health by using a metagenomics approach.
|Title||FBIP: Cataloguing zoonotic tick-borne bacterial pathogen strain diversity in wild rodent species in rural South Africa|
|Funding||Foundational Biodiversity Information Programme|
|Study Area Description||South Africa (Mpumalanga)|
The personnel involved in the project:
-Ethical clearance through the Animal Ethics Committee (AEC) of the University of Pretoria was obtained (V046-14 and V030-14). -302 rodent blood samples across three habitat types (urban/periurban, communal rangelands, adjacent protected rangeland areas) in Mnisi has already been collected. Further Trapping will be done using Sherman-type traps; five sites will be pre-selected per habitat type; 50 traps will be used/site for up to 5 consecutive days. Traps will be baited with peanut butter, oats and marmite, checked twice/day. We aim to trap at least 15-20 individuals of 10 main species of rodents in each of the habitat types (n=600). Targeted species will be euthanized, blood will be collected immediately after euthanasian by cardiocentesis. Carcasses will be placed individually in sealed plastic bags and frozen.
|Study Extent||South Africa (Mpumalanga) in Mnisi|
Method step description:
- -Apart from identifying rodent species by means of the Stuarts’ Field Guide to Mammals of Southern Africa (Stuart & Stuart, 2001), species verification will be done using sequence analysis of the mitochondrial gene Cytochrome Oxidase I (COI) -Catalogue the occurrence of the tick-borne bacterial pathogens present in wild rodent species by DNA analysis of the rodent microbiome. -Establish the genetic diversity of A. phagocytophilum strains by using specific gene markers.