FBIP: What are the impacts of global climate change on arid soil microbial communities?

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 3,986 records.

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How to cite

Researchers should cite this work as follows:

Makhalanyane T (2020): FBIP: What are the impacts of global climate change on arid soil microbial communities?. v1.1. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=what&v=1.1

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is South African National Biodiversity Institute. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: a387a0b3-7cf8-4d73-8851-eccbd882e16d.  South African National Biodiversity Institute publishes this resource, and is itself registered in GBIF as a data publisher endorsed by South African Biodiversity Information Facility.

Keywords

Occurrence; Specimen

Contacts

Geographic Coverage

South Africa (Northern Cape, KwaZulu Natal, Free State)

Taxonomic Coverage

A broad survey of microbial (bacteria, archaea, fungi and protista) communities along a West-East aridity gradient across South Africa.

Temporal Coverage

Project Data

A broad survey of microbial (bacteria, archaea, fungi and protista) communities along a West-East aridity gradient across South Africa.

The personnel involved in the project:

Sampling Methods

Whole animals where collected

Method step description:

1. Samples will be collected during a field campaign in early 2016, along an aridity gradient (Figure 1: Attachment). The aridity gradient will be sampled from a single lithostratigraphic group (Volksrust Group) to minimise variation due to geology. The landform stretches from West to East across central South Africa, and experiences rainfall, which ranges from approximately 100 mm p.a. to 1000 mm p.a. It mainly runs through the Nama Karoo and the Grassland biomes. One hundred and sixty sampling sites will be selected at regular intervals across the rainfall gradient. These will be identified with the help of national spatial datasets to ensure that the aridity gradient is evenly sampled. Best estimates of annual rainfall for each collecting site will be extracted from national climatological datasets. DNA from the soil samples will be extracted using well-established protocols (13), and subjected to amplicon sequencing analysis as specified in WP 2 (see detailed below). In order to obtain phylogenetic information, tagged amplicon pyrosequencing will be done using known markers. Soil chemistry and respiration assays will be conducted (see detailed below) in order to quantify the levels of carbon along the aridity gradient.

Additional Metadata