Ocorrência

FBIP: DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies

Versão mais recente publicado por South African National Biodiversity Institute em 28 de Junho de 2019 South African National Biodiversity Institute
Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher sp... Mais
Publication date:
28 de Junho de 2019
License:
CC-BY 4.0

Descrição

Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher specimens collected from two localities, their identities, and DNA sequences. The voucher specimens have been deposited in the Ria Olivier Herbarium at NMMU.

Registros de Dados

Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 357 registros.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Downloads

Baixe a última versão do recurso de dados, como um Darwin Core Archive (DwC-A) ou recurso de metadados, como EML ou RTF:

Dados como um arquivo DwC-A download 357 registros em English (25 kB) - Frequência de atualização: desconhecido
Metadados como um arquivo EML download em English (12 kB)
Metadados como um arquivo RTF download em English (11 kB)

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Kerley G (2019): FBIP: DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=dna_barcoding&v=1.0

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é South African National Biodiversity Institute. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 2dd5d605-ce02-4496-bbba-b4723a3709c0.  South African National Biodiversity Institute publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por South African Biodiversity Information Facility.

Palavras-chave

Addo Elephant National Park; herbivore diet reference collection for DNA barcoding; Specimen

Contatos

Quem criou esse recurso:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

Quem pode responder a perguntas sobre o recurso:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

Quem preencher os metadados:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

Quem mais foi associado com o recurso:

Provedor de Conteúdo
Mahlatse Kgatla
FBIP Data Specialist
SANBI
2 Cussonia Avenue, Brummeria
0184 Pretoria
Gauteng
ZA
0128435196
http://fbip.co.za/contact/

Cobertura Geográfica

Addo Elephant National Park, two localities: Main Camp and Colchester

Coordenadas delimitadoras Sul Oeste [-33,631, 25,703], Norte Leste [-33,442, 25,917]

Cobertura Taxonômica

Most specimen have been identified to Genus level while others to Species and Subspecies level.

Reino  Plantae

Cobertura Temporal

Data Inicial 2014-01-01

Dados Sobre o Projeto

Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher specimens collected from two localities, their identities, and DNA sequences. The voucher specimens have been deposited in the Ria Olivier Herbarium at NMMU.

Título DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies
Identificador IBIP-BS13100452303
Financiamento Foundational Biodiversity Information Programme
Descrição da Área de Estudo Addo Elephant National Park, two localities: Main Camp and Colchester

O pessoal envolvido no projeto:

Pesquisador Principal
Graham Kerley

Métodos de Amostragem

Data from specimen labels / datasheets. DNA barcoding done by Prof P Taberlet of Centre National de la Recherche Scientifique, France. Specimens vouchered in Ria Olivier Herbarium, identified by PhD student (C. Weatherall-Thomas), Prof E. Campbell. Specimens that could not be identified based on morphology were barcoded and barcodes used to obtain identifications

Área de Estudo Addo Elephant National Park, two localities: Main Camp and Colchester

Descrição dos passos do método:

  1. This survey will be undertaken throughout the Addo Main Camp and Colchester Sections of the Addo Elephant National Park, this to cover 8 vegetation types that occur here. An initial literature and expert-based survey indicates that approximately 500 plant species should be sampled, these reflecting both dominant plant species, plant species of conservation significance and plants that are potentially consumed by the herbivores (the latter comprising 13 species) Sampling will comprise the collection of tissue and herbarium samples. Samples will be located in the field by local experts, tissues samples stored in labelled, silica-dried tubes and matched plant species will be prepared for herbarium curation in the Ria Olivier Herbarium at the Nelson Mandela Metropolitan University. This phase (plant collection, identification and curation) will be undertaken by a team of botanists and ecologists, including Profs Campbell and Kerley, Dr Landman together with postgraduate and undergraduate students. SANParks Regional Ecologist A Gaylard will also assist with this phase, together with SANParks Field Rangers (for protection of researchers). The field work will be initiated in January 2014 (or as soon as funds are released) and will run for 12 months. In this period there will be at least three major sampling trips to cover the phenological patterns of the plant communities. The tissue samples will then be sent to France, under a BABS permit, for analysis. The DNA barcoding itself will be supported by a separate project and will be undertaken in collaboration with Dr Pierre Taberlet of the Joseph Fourier University, France. The DNA analysis will be divided into three sub-tasks: (i) DNA extraction, (ii) DNA amplification, and (iii) DNA sequencing. Sequence reads will be analysed using tools specifically designed for such analyses, including http://www.grenoble.prabi.fr/trac/OBITools. It is anticipated that these DNA analyses will require an additional 6 months.

Metadados Adicionais