Occurrence

FBIP: DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies

Dernière version Publié par South African National Biodiversity Institute le 28 juin 2019 South African National Biodiversity Institute
Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher sp... plus
Date de publication:
28 juin 2019
Licence:
CC-BY 4.0

Description

Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher specimens collected from two localities, their identities, and DNA sequences. The voucher specimens have been deposited in the Ria Olivier Herbarium at NMMU.

Enregistrements de données

Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 357 enregistrements.

Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.

Téléchargements

Téléchargez la dernière version de la ressource en tant qu'Archive Darwin Core (DwC-A), ou les métadonnées de la ressource au format EML ou RTF :

Données sous forme de fichier DwC-A (zip) télécharger 357 enregistrements dans Anglais (25 kB) - Fréquence de mise à jour: inconnue
Métadonnées sous forme de fichier EML télécharger dans Anglais (12 kB)
Métadonnées sous forme de fichier RTF télécharger dans Anglais (11 kB)

Versions

Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.

Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Kerley G (2019): FBIP: DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=dna_barcoding&v=1.0

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est South African National Biodiversity Institute. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 2dd5d605-ce02-4496-bbba-b4723a3709c0.  South African National Biodiversity Institute publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du South African Biodiversity Information Facility.

Mots-clé

Addo Elephant National Park; herbivore diet reference collection for DNA barcoding; Specimen

Contacts

Personne ayant créé cette ressource:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

Personne pouvant répondre aux questions sur la ressource:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

Personne ayant renseigné les métadonnées:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

Autres personnes associées à la ressource:

Fournisseur de Contenu
Mahlatse Kgatla
FBIP Data Specialist
SANBI
2 Cussonia Avenue, Brummeria
0184 Pretoria
Gauteng
ZA
0128435196
http://fbip.co.za/contact/

Couverture géographique

Addo Elephant National Park, two localities: Main Camp and Colchester

Enveloppe géographique Sud Ouest [-33,631, 25,703], Nord Est [-33,442, 25,917]

Couverture taxonomique

Most specimen have been identified to Genus level while others to Species and Subspecies level.

Kingdom  Plantae

Couverture temporelle

Date de début 2014-01-01

Données sur le projet

Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher specimens collected from two localities, their identities, and DNA sequences. The voucher specimens have been deposited in the Ria Olivier Herbarium at NMMU.

Titre DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies
Identifiant IBIP-BS13100452303
Financement Foundational Biodiversity Information Programme
Description du domaine d'étude / de recherche Addo Elephant National Park, two localities: Main Camp and Colchester

Les personnes impliquées dans le projet:

Chercheur Principal
Graham Kerley

Méthodes d'échantillonnage

Data from specimen labels / datasheets. DNA barcoding done by Prof P Taberlet of Centre National de la Recherche Scientifique, France. Specimens vouchered in Ria Olivier Herbarium, identified by PhD student (C. Weatherall-Thomas), Prof E. Campbell. Specimens that could not be identified based on morphology were barcoded and barcodes used to obtain identifications

Etendue de l'étude Addo Elephant National Park, two localities: Main Camp and Colchester

Description des étapes de la méthode:

  1. This survey will be undertaken throughout the Addo Main Camp and Colchester Sections of the Addo Elephant National Park, this to cover 8 vegetation types that occur here. An initial literature and expert-based survey indicates that approximately 500 plant species should be sampled, these reflecting both dominant plant species, plant species of conservation significance and plants that are potentially consumed by the herbivores (the latter comprising 13 species) Sampling will comprise the collection of tissue and herbarium samples. Samples will be located in the field by local experts, tissues samples stored in labelled, silica-dried tubes and matched plant species will be prepared for herbarium curation in the Ria Olivier Herbarium at the Nelson Mandela Metropolitan University. This phase (plant collection, identification and curation) will be undertaken by a team of botanists and ecologists, including Profs Campbell and Kerley, Dr Landman together with postgraduate and undergraduate students. SANParks Regional Ecologist A Gaylard will also assist with this phase, together with SANParks Field Rangers (for protection of researchers). The field work will be initiated in January 2014 (or as soon as funds are released) and will run for 12 months. In this period there will be at least three major sampling trips to cover the phenological patterns of the plant communities. The tissue samples will then be sent to France, under a BABS permit, for analysis. The DNA barcoding itself will be supported by a separate project and will be undertaken in collaboration with Dr Pierre Taberlet of the Joseph Fourier University, France. The DNA analysis will be divided into three sub-tasks: (i) DNA extraction, (ii) DNA amplification, and (iii) DNA sequencing. Sequence reads will be analysed using tools specifically designed for such analyses, including http://www.grenoble.prabi.fr/trac/OBITools. It is anticipated that these DNA analyses will require an additional 6 months.

Métadonnées additionnelles