Registros biológicos

FBIP: DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies

Última versión Publicado por South African National Biodiversity Institute en 28 de junio de 2019 South African National Biodiversity Institute
Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher sp... Más
Fecha de publicación:
28 de junio de 2019
Licencia:
CC-BY 4.0

Descripción

Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher specimens collected from two localities, their identities, and DNA sequences. The voucher specimens have been deposited in the Ria Olivier Herbarium at NMMU.

Registros

Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 357 registros.

Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.

Descargas

Descargue la última versión de los datos como un Archivo Darwin Core (DwC-A) o los metadatos como EML o RTF:

Datos como un archivo DwC-A descargar 357 registros en Inglés (25 kB) - Frecuencia de actualización: desconocido
Metadatos como un archivo EML descargar en Inglés (12 kB)
Metadatos como un archivo RTF descargar en Inglés (11 kB)

Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Kerley G (2019): FBIP: DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=dna_barcoding&v=1.0

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es South African National Biodiversity Institute. Este trabajo está autorizado bajo una Licencia Creative Commons Atribución/Reconocimiento 4.0 Internacional (CC-BY) 4.0.

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 2dd5d605-ce02-4496-bbba-b4723a3709c0.  South African National Biodiversity Institute publica este recurso y está registrado en GBIF como un publicador de datos avalado por South African Biodiversity Information Facility.

Palabras clave

Addo Elephant National Park; herbivore diet reference collection for DNA barcoding; Specimen

Contactos

¿Quién creó el recurso?:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

¿Quién puede resolver dudas acerca del recurso?:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

¿Quién documentó los metadatos?:

Graham Kerley
Professor and Director of Centre for African Conservation Ecology
Nelson Mandela Metropolitan University
Summerstrand Campus (South), Department of Zoology, Room 12-114c
Port Elizabeth
Eastern Cape
ZA
+27 41 504 2308
http://ace.mandela.ac.za/

¿Quién más está asociado con el recurso?:

Proveedor de Contenido
Mahlatse Kgatla
FBIP Data Specialist
SANBI
2 Cussonia Avenue, Brummeria
0184 Pretoria
Gauteng
ZA
0128435196
http://fbip.co.za/contact/

Cobertura geográfica

Addo Elephant National Park, two localities: Main Camp and Colchester

Coordenadas límite Latitud Mínima Longitud Mínima [-33,631, 25,703], Latitud Máxima Longitud Máxima [-33,442, 25,917]

Cobertura taxonómica

Most specimen have been identified to Genus level while others to Species and Subspecies level.

Reino  Plantae

Cobertura temporal

Fecha Inicial 2014-01-01

Datos del proyecto

Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher specimens collected from two localities, their identities, and DNA sequences. The voucher specimens have been deposited in the Ria Olivier Herbarium at NMMU.

Título DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies
Identificador IBIP-BS13100452303
Fuentes de Financiación Foundational Biodiversity Information Programme
Descripción del área de estudio Addo Elephant National Park, two localities: Main Camp and Colchester

Personas asociadas al proyecto:

Investigador Principal
Graham Kerley

Métodos de muestreo

Data from specimen labels / datasheets. DNA barcoding done by Prof P Taberlet of Centre National de la Recherche Scientifique, France. Specimens vouchered in Ria Olivier Herbarium, identified by PhD student (C. Weatherall-Thomas), Prof E. Campbell. Specimens that could not be identified based on morphology were barcoded and barcodes used to obtain identifications

Área de Estudio Addo Elephant National Park, two localities: Main Camp and Colchester

Descripción de la metodología paso a paso:

  1. This survey will be undertaken throughout the Addo Main Camp and Colchester Sections of the Addo Elephant National Park, this to cover 8 vegetation types that occur here. An initial literature and expert-based survey indicates that approximately 500 plant species should be sampled, these reflecting both dominant plant species, plant species of conservation significance and plants that are potentially consumed by the herbivores (the latter comprising 13 species) Sampling will comprise the collection of tissue and herbarium samples. Samples will be located in the field by local experts, tissues samples stored in labelled, silica-dried tubes and matched plant species will be prepared for herbarium curation in the Ria Olivier Herbarium at the Nelson Mandela Metropolitan University. This phase (plant collection, identification and curation) will be undertaken by a team of botanists and ecologists, including Profs Campbell and Kerley, Dr Landman together with postgraduate and undergraduate students. SANParks Regional Ecologist A Gaylard will also assist with this phase, together with SANParks Field Rangers (for protection of researchers). The field work will be initiated in January 2014 (or as soon as funds are released) and will run for 12 months. In this period there will be at least three major sampling trips to cover the phenological patterns of the plant communities. The tissue samples will then be sent to France, under a BABS permit, for analysis. The DNA barcoding itself will be supported by a separate project and will be undertaken in collaboration with Dr Pierre Taberlet of the Joseph Fourier University, France. The DNA analysis will be divided into three sub-tasks: (i) DNA extraction, (ii) DNA amplification, and (iii) DNA sequencing. Sequence reads will be analysed using tools specifically designed for such analyses, including http://www.grenoble.prabi.fr/trac/OBITools. It is anticipated that these DNA analyses will require an additional 6 months.

Metadatos adicionales