FBIP: Stellenbosch University: Freshwater Prawns Survey

出現紀錄
最新版本 published by South African National Biodiversity Institute on 九月 30, 2019 South African National Biodiversity Institute
發布日期:
2019年9月30日
授權條款:
CC-BY 4.0

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說明

Distribution of fresh water prawns in the Eastern Cape and KwaZulu Natal rivers.

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如何引用

研究者應依照以下指示引用此資源。:

Daniels P S R (2019): FBIP: Stellenbosch University: Freshwater Prawns Survey. v1.1. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=freshwater_prawns_survey&v=1.1

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此資料的發布者及權利單位為 South African National Biodiversity Institute。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

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關鍵字

Occurrence

聯絡資訊

Prof Savel R Daniels
  • 元數據提供者
  • 出處
  • 連絡人
Professor of Zoology
Stellenbosch University
Natural Sciences, Botany and Zoology, P.O. Box X1,Matieland
7602 Stellenbosch
Western Cape
+27218083230

地理涵蓋範圍

Eastern Cape and KwaZulu-Natal

界定座標範圍 緯度南界 經度西界 [-34.34, 18.008], 緯度北界 經度東界 [-27.362, 32.535]

分類群涵蓋範圍

Freshwater prawns

Order Decapoda

時間涵蓋範圍

起始日期 / 結束日期 2016-01-01 / 2016-03-31

取樣方法

Sample collection and field work: The current survey project is designed to run over a two year period (2015 -2016). Given the extensive geographic scope of the project and the large number of rivers to be sampled it can only be practically feasible to undertake the survey over a two year period. During the current year, 2015 the Eastern Cape province rivers will be sampled, these include the following, Diep, Gamtoos, Krom, Bushmans, Kowie, Kap, Great Fish, Keiskamma, Laing, Nahoon Dam, Great Fish and Kei. During the following year, 2016, KwaZulu-Natal rivers will be sampled, these include the following rivers, Mthatha, Mzimvula, Mtentu, Mtamvuna, Mzimkulu, Mkomazi, Mgeni, Mvoti,uThukela, Mfolozi, Nyalazi, Msunduzo, Mkuzi and Pongola. At each site we will sample three localities (upper, middle and lower reaches) taking a minimum of ten samples per sample locality (yielding an estimated total of 780 prawn specimens). Samples will be collected, using baited traps, hand nets or trawling - depending on the depth of the river. Traps baited with fish or dog food will be left on site for 24 hours. Upon collection of samples, GPS coordinates will be taken and the specimens will be photographed using a Nikon Digital Camera. Samples will be euthanized with clove oil, and preserved in absolute ethanol in honey jars. Samples will be identified using keys together with the help of Dr De Grave, a leadings specialist on freshwater prawns. Upon completion of the sample collection and the data analyses, specimens will be deposited in the South African Natural History Museum collection (Iziko) of Cape Town. It is important to note that I have already sampled all the major rivers in the Western Cape province together with my PhD student. This work forms a natural extension of our sampling effort and all the data will be used towards her PhD thesis. (It is very important to note that we are not planning to sample the remaining provinces at this point because there is a cap on the feasibility of a survey that will include all of the remaining provinces in the country).

研究範圍 Eastern Cape and KwaZulu-Natal

方法步驟描述:

  1. DNA extraction: DNA will be extracted using a Machery and Nagel kit following the extraction protocol of the manufacturer. DNA will be stored in a refrigerator prior to use. We will amplify three loci. These include the barcoding locus cytochrome oxidase one (COI) and two nuclear markers, histone three and 28S. Universal primer pairs will be obtained from the literature and are currently already being used by my PhD student Ms. Wood who works on prawns in the Western Cape. PCR's will be done in a thermocycler and the PCR products will be gel purified using a Bioflux gel purification kit. Samples will then be sent to the central analytical facility at Stellenbosch University for DNA sequencing. This aspect of the study will be done after each field trip and is estimated to take about six months. All of the sequences will be deposited in GenBank / BOLD. Molecular data analyses: Sequence data will be aligned in MUSCLE and the two protein coding markers (COI and histone 3) will be checked for stop codons. It should be noted that nuclear DNA markers in Arthropods are generally slow evolving so we will explore a number of variable marker options - however, these will in the end be restricted to two loci (as per our current budget).We will collapse all the COI data into networks for each of the species using TCS. An SAMOVA and AMOVA will be performed on the COI data. We are selecting this locus because it is the most rapidly evolving locus we will use. In addition we will also construct phylogenetic trees for the haplotypes for each respective species using Bayesian inference and Maximum Likelihood. This aspect of the study is estimated to take four months. Quantification of samples for in situ fisheries: The larger Macrobrachium species have real potential for small scale in situ fisheries and we will focus on these species to estimate their abundance. Total abundance and biomass of prawns will be calculated using the swept area method. The area sampled will be calculated by multiplying the effective width of the trawl by the towing distance. To standardize data, the number of individuals or the total wet weight of the individuals captured at a sampling station will be divided by the area sampled. A mean abundance and biomass of the sampled stations will then be calculated. Abundance results will be expressed as individuals per m square, and biomass in grams wet weight per m square. Wet weight will be measured using a Sauter AR100 microbalance. This activity will happen at every sample site where Macrobrachium is collected.