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版本
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如何引用
研究者應依照以下指示引用此資源。:
Oosthuizen M (2020): FBIP: Cataloguing zoonotic tick-borne bacterial pathogen strain diversity in wild rodent species in rural South Africa. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=fbip2&v=1.0
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 South African National Biodiversity Institute。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
GBIF 註冊
此資源已向GBIF註冊,並指定以下之GBIF UUID: eb8708e4-5788-4c44-89c9-ef54e4423086。 South African National Biodiversity Institute 發佈此資源,並經由South African Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence; Specimen
聯絡資訊
資源建立者:
可回覆此資源相關問題者:
元數據填寫者:
與此資源的相關者:
地理涵蓋範圍
South Africa (Mpumalanga)
界定座標範圍 | 緯度南界 經度西界 [-24.827, 31.33], 緯度北界 經度東界 [-24.617, 32.371] |
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分類群涵蓋範圍
Catalogue the tick-borne bacterial pathogen strain diversity present in wild rodent species
Genus | Anaplasma (Bacteria), Mastomys (Rodents) |
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時間涵蓋範圍
起始日期 / 結束日期 | 2012-04-01 / 2016-04-05 |
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計畫資料
The aim of the study is, therefore, to catalogue the tick-borne bacterial pathogen strain diversity present in wild rodent species in the Mnisi community that could impact human health by using a metagenomics approach.
計畫名稱 | FBIP: Cataloguing zoonotic tick-borne bacterial pathogen strain diversity in wild rodent species in rural South Africa |
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辨識碼 | FBIS170330225236 |
經費來源 | Foundational Biodiversity Information Programme |
研究區域描述 | South Africa (Mpumalanga) |
參與計畫的人員:
取樣方法
-Ethical clearance through the Animal Ethics Committee (AEC) of the University of Pretoria was obtained (V046-14 and V030-14). -302 rodent blood samples across three habitat types (urban/periurban, communal rangelands, adjacent protected rangeland areas) in Mnisi has already been collected. Further Trapping will be done using Sherman-type traps; five sites will be pre-selected per habitat type; 50 traps will be used/site for up to 5 consecutive days. Traps will be baited with peanut butter, oats and marmite, checked twice/day. We aim to trap at least 15-20 individuals of 10 main species of rodents in each of the habitat types (n=600). Targeted species will be euthanized, blood will be collected immediately after euthanasian by cardiocentesis. Carcasses will be placed individually in sealed plastic bags and frozen.
研究範圍 | South Africa (Mpumalanga) in Mnisi |
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品質控管 | NA |
方法步驟描述:
- -Apart from identifying rodent species by means of the Stuarts’ Field Guide to Mammals of Southern Africa (Stuart & Stuart, 2001), species verification will be done using sequence analysis of the mitochondrial gene Cytochrome Oxidase I (COI) -Catalogue the occurrence of the tick-borne bacterial pathogens present in wild rodent species by DNA analysis of the rodent microbiome. -Establish the genetic diversity of A. phagocytophilum strains by using specific gene markers.