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版本
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如何引用
研究者應依照以下指示引用此資源。:
Jacobs A (2019): FBIP: Barcoding of the national collection of fungi: with special Emphasis on Hypocrealean fungi. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=barcoding&v=1.0
權利
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此資料的發布者及權利單位為 South African National Biodiversity Institute。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: b5cd47e0-0039-4092-a77f-7493b586a3e1。 South African National Biodiversity Institute 發佈此資源，並經由South African Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。
關鍵字
Barcoding; Hypocrealean; Fungi; DNA; BLAST; Specimen
聯絡資訊
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可回覆此資源相關問題者:
元數據填寫者:
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地理涵蓋範圍
Limpopo, Norther West, Gauteng, Mpumalanga, KwaZulu Natal, Eastern Cape, Free State, Western Cape
| 界定座標範圍 | 緯度南界 經度西界 [-34.886, 16.348], 緯度北界 經度東界 [-22.106, 32.959] |
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分類群涵蓋範圍
Most specimen have been identified to Genus and Species level, some are identified to Phylum and Class level
| Phylum | Fungi |
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時間涵蓋範圍
| 起始日期 / 結束日期 | 1993-06-01 / 2017-07-23 |
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計畫資料
The main aim of this study is to expand the current knowledge basis on soil fungi by generating DNA barcodes for isolates in the NCF that has not been identified to species level as they could represent new species. The focus will be on the large portion of strains. representing the plant pathogenic and beneficial genera Fusarium and Trichoderma, respectively.
| 計畫名稱 | Barcoding of the national collection of fungi: with special Emphasis on Hypocrealean fungi |
|---|---|
| 辨識碼 | FBIS150520118174 |
| 經費來源 | Funding from Foundational Biodiversity Information Programme (FBIP) |
| 研究區域描述 | Limpopo, Norther West, Gauteng, Mpumalanga, KwaZulu Natal, Eastern Cape, Free State, Western Cape |
參與計畫的人員:
取樣方法
samples where sourced from Agricultural Reasearch Council collection of living specimens.
| 研究範圍 | South Africa |
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方法步驟描述:
- Strain selection: Strains representing the order Hypocreales will be selected based on the following criteria: a)No Sequence data available for the strain b) Morphological identification done to genus level or higher and c) Isolate obtained from soil . These will include strains from the MRC PROMEC collection as the ARC is now the custodians of this world renowned collection of fungi of medical importance. Amplicon generation: DNA extractions and amplification of target gene. The target gene will be genus specific as the ITS gene region doesn't provide species level identification for this group of fungi. For the genera Fusarium and Trichoderma it will be the translation elongation factor 1a gene as (Geiser et al., 2004; European Journal of Plant Pathology 110: 473-479; O’ Donnell et al., 2012; Mycologia, 104: 427–445; Druzhinina et al., Microbiology (2008), 154, 3447–3459. Data analyses: BLAST analyses will be done on the obtained sequence data using a number of international mycological databases such as TrichoMARK, Fusarium MLST and Fusarium ID, MycoBank and NCBI GenBank. The DNA based identification will be used to update the information in the NCF's database. This will also ensure that data is available on these selected strains that would be incorporated in taxonomic re-evaluations of orders, species complexes etc., and will be available for research sectors in agriculture, health, and industry. Population of databases: The newly generated data will be incorporate in the soil and phytopathogenic databases' BLAST tool which will also consult existing data in CBS, NCF and NCBI GenBank. The data will also be deposited in the BOLD database. Collaborative research: The barcoded species will be included in taxonomic and phylogenetic revisions that are currently being performed by the researchers located at the NCF (ARC-PPR) and both national and internationally.