http://ipt.sanbi.org.za/iptsanbi/resource?r=what FBIP: What are the impacts of global climate change on arid soil microbial communities? Thulani Makhalanyane University of Pretoria Lecturer
Natural Sciences 2, Office 3-14, Hatfied Campus, Lynwood Road Pretoria Gauteng 0028 ZA
012 4206976 thulani.makhalanyane@up.ac.za
Thulani Makhalanyane University of Pretoria Lecturer
Natural Sciences 2, Office 3-14, Hatfied Campus, Lynwood Road Pretoria Gauteng 0028 ZA
012 4206976 thulani.makhalanyane@up.ac.za
Mahlatse Kgatla SANBI FBIP Data Specialist
2 Cussonia Avenue Pretoria Gauteng 0184 ZA
0128435196 m.kgatla@sanbi.org.za http://fbip.co.za/contact/ contentProvider
2020-06-30 eng A broad survey of microbial (bacteria, archaea, fungi and protista) communities along a West-East aridity gradient across South Africa. Occurrence GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml Specimen GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. South Africa (Northern Cape, KwaZulu Natal, Free State) 16.348 29.839 -24.807 -34.814 2016-02-19 2016-02-23 A broad survey of microbial (bacteria, archaea, fungi and protista) communities along a West-East aridity gradient across South Africa. kingdom Fungi kingdom Archaea kingdom Bacteria kingdom Protista unkown Thulani Makhalanyane University of Pretoria Lecturer
Natural Sciences 2, Office 3-14, Hatfied Campus, Lynwood Road Pretoria Gauteng 0028 ZA
012 4206976 thulani.makhalanyane@up.ac.za
Samples will be collected during a field campaign in early 2016, along an aridity gradient (Figure 1: Attachment). The aridity gradient will be sampled from a single lithostratigraphic group (Volksrust Group) to minimise variation due to geology. The landform stretches from West to East across central South Africa, and experiences rainfall, which ranges from approximately 100 mm p.a. to 1000 mm p.a. It mainly runs through the Nama Karoo and the Grassland biomes. One hundred and sixty sampling sites will be selected at regular intervals across the rainfall gradient. These will be identified with the help of national spatial datasets to ensure that the aridity gradient is evenly sampled. Best estimates of annual rainfall for each collecting site will be extracted from national climatological datasets. DNA from the soil samples will be extracted using well-established protocols (13), and subjected to amplicon sequencing analysis as specified in WP 2 (see detailed below). In order to obtain phylogenetic information, tagged amplicon pyrosequencing will be done using known markers. Soil chemistry and respiration assays will be conducted (see detailed below) in order to quantify the levels of carbon along the aridity gradient. South Africa (Northern Cape, KwaZulu Natal, Free State) Whole animals where collected What are the impacts of global climate change on arid soil microbial communities? Thulani Makhalanyane principalInvestigator A broad survey of microbial (bacteria, archaea, fungi and protista) communities along a West-East aridity gradient across South Africa. Foundational Biodiversity Information Programme South Africa (Northern Cape, KwaZulu Natal, Free State)
2020-06-29T06:33:52.883+02:00 dataset Makhalanyane T (2020): FBIP: What are the impacts of global climate change on arid soil microbial communities?. v1.1. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=what&v=1.1 http://ipt.sanbi.org.za/iptsanbi/resource?id=what/v1.1.xml