http://ipt.sanbi.org.za/iptsanbi/resource?r=fbip2 FBIP: Cataloguing zoonotic tick-borne bacterial pathogen strain diversity in wild rodent species in rural South Africa Marinda Oosthuizen University of Pretoria Professor
Faculty of Veterinary Science, Arnold Theiler Building, Room 2.20-3, Hatfied Campus, Soutpan Road (M35) Pretoria Gauteng 0110 ZA
0125298390 marinda.oosthuizen@up.ac.za
Marinda Oosthuizen University of Pretoria Professor
Faculty of Veterinary Science, Arnold Theiler Building, Room 2.20-3, Hatfied Campus, Soutpan Road (M35) Pretoria Gauteng 0110 ZA
0125298390 marinda.oosthuizen@up.ac.za
Mahlatse Kgatla SANBI FBIP Data Specialist
2 Cussonia Avenue, Brummeria Pretoria Gauteng 0184 ZA
0128435196 m.kgatla@sanbi.org.za http://fbip.co.za/contact/ distributor
2020-09-30 eng The aim of the study is, therefore, to catalogue the tick-borne bacterial pathogen strain diversity present in wild rodent species in the Mnisi community that could impact human health by using a metagenomics approach. Occurrence GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml Specimen GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. South Africa (Mpumalanga) 31.33 32.371 -24.617 -24.827 2012-04-01 2016-04-05 Catalogue the tick-borne bacterial pathogen strain diversity present in wild rodent species genus Anaplasma Bacteria genus Mastomys Rodents unkown Marinda Oosthuizen University of Pretoria Professor
Faculty of Veterinary Science, Arnold Theiler Building, Room 2.20-3, Hatfied Campus, Soutpan Road (M35) Pretoria Gauteng 0110 ZA
0125298390 marinda.oosthuizen@up.ac.za
-Apart from identifying rodent species by means of the Stuarts’ Field Guide to Mammals of Southern Africa (Stuart & Stuart, 2001), species verification will be done using sequence analysis of the mitochondrial gene Cytochrome Oxidase I (COI) -Catalogue the occurrence of the tick-borne bacterial pathogens present in wild rodent species by DNA analysis of the rodent microbiome. -Establish the genetic diversity of A. phagocytophilum strains by using specific gene markers. South Africa (Mpumalanga) in Mnisi -Ethical clearance through the Animal Ethics Committee (AEC) of the University of Pretoria was obtained (V046-14 and V030-14). -302 rodent blood samples across three habitat types (urban/periurban, communal rangelands, adjacent protected rangeland areas) in Mnisi has already been collected. Further Trapping will be done using Sherman-type traps; five sites will be pre-selected per habitat type; 50 traps will be used/site for up to 5 consecutive days. Traps will be baited with peanut butter, oats and marmite, checked twice/day. We aim to trap at least 15-20 individuals of 10 main species of rodents in each of the habitat types (n=600). Targeted species will be euthanized, blood will be collected immediately after euthanasian by cardiocentesis. Carcasses will be placed individually in sealed plastic bags and frozen. NA FBIP: Cataloguing zoonotic tick-borne bacterial pathogen strain diversity in wild rodent species in rural South Africa Marinda Oosthuizen principalInvestigator The aim of the study is, therefore, to catalogue the tick-borne bacterial pathogen strain diversity present in wild rodent species in the Mnisi community that could impact human health by using a metagenomics approach. Foundational Biodiversity Information Programme South Africa (Mpumalanga)
2020-09-22T02:56:39.759+02:00 dataset Oosthuizen M (2020): FBIP: Cataloguing zoonotic tick-borne bacterial pathogen strain diversity in wild rodent species in rural South Africa. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=fbip2&v=1.0