http://ipt.sanbi.org.za/iptsanbi/resource?r=taxonomy FBIP: Taxonomy and Candidatus Liberibacter status of South African Buchu plants Gerhard Pietersen Agricultural Research Council - Plant Protection Research Institute Extra-ordinary Professor
Room 9-21, New Agricultural Building, Lunnon Street, University of Pretoria Pretoria Gauteng 0002 ZA
012 4203265 gerhard.pietersen@up.ac.za
Gerhard Pietersen Agricultural Research Council - Plant Protection Research Institute Extra-ordinary Professor
Room 9-21, New Agricultural Building, Lunnon Street, University of Pretoria Pretoria Gauteng 0002 ZA
012 4203265 gerhard.pietersen@up.ac.za
Mahlatse Kgatla SANBI FBIP Data Specialist
2 Cussonia Avenue Pretoria Gauteng 0184 ZA
0128435196 m.kgatla@sanbi.org.za https://fbip.co.za/contact/ contentProvider
2020-06-30 eng Survey of Rutaceae species of the fynbos area. Additional data, will help provide abundance lists, distribution and locality of these plants, while the molecular means to identify the species. Occurrence GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml Specimen GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. South Africa (Western Cape, Kwa-Zulu Natal) 17.71 33.223 -26.746 -34.778 2014-10-16 2018-01-12 Family: Rutaceae family Rutaceae Buchus Plants unkown Gerhard Pietersen Agricultural Research Council - Plant Protection Research Institute Extra-ordinary Professor
Room 9-21, New Agricultural Building, Lunnon Street, University of Pretoria Pretoria Gauteng 0002 ZA
012 4203265 gerhard.pietersen@up.ac.za
1) We plan to collect and sequence barcoding genes of known, morphologically identified specimens of buchu collected under permit at Kirstenbosch botanical gardens or from herbarium specimens where permission is granted to do so. Collection from live plants will not be destructive as only about 5g fresh weight of leaf material is required in order to obtain phloem tissue (to which the Ca. Liberibacters are confined). 2) We will collect leaf material from large numbers of buchu specimens (we plan to collect a total of about 1000 specimens in total, with between 10 and 20 replicates of visually identified species of “buchu” (in the broadest sense) from randomly selected transects from three Western Cape nature reserves containing the greatest diversity of buchu, and where permits for the study can be obtained. This will be done in the flowering season of two successive years. All samples obtained will be assigned a unique accession number and the following data will be recorded for each specimen: date of collection, collector details, photographs of the plant and any distinguishing feature(s), plant visual identification, locality data, the exact location (to an accuracy of 0.25m determined by a differential GPS) and the habitat description. 3) Collected material will be archived in the South African Plant Virus culture collection (SAPVC) at ARC-PPRI, as desiccated plant material as well as in -80oC freezer storage, and voucher specimens for identification purposes submitted to the Compton Herbarium (NBG) and National Herbarium (PRE). 5) All samples will be tested for Ca. Liberibacter using a generic Ca. Liberibacter PCR developed in our laboratory (Pietersen, unpublished), based on all known Ca. Liberibacter sequences. 6) All Liberibacter infected samples (as determined by real-time PCR) will be tested with primers to specific Liberibacters (Ca. Liberibacter -americanus, -africanus, - asiaticus, -solanacearum and -africanus subspecies –capensis, -verpidis, -clausenae, -zanthoxyli, and –tecleae) in conventional PCR’s or a newly developed multiplex PCR capable of detecting these (Roberts unpublished results). 7) All plant samples, irrespective of Ca. Liberibacter status, will be subjected to barcoding PCR’s (rbcL, matK, trnH/psb) and the amplicons sequenced by Sanger sequencing. South Africa (Western Cape, Kwa-Zulu Natal) Leaves were collected Taxonomy and Candidatus Liberibacter status of South African Buchu plants Gerhard Pietersen principalInvestigator Survey of Rutaceae species of the fynbos area. Additional data, will help provide abundance lists, distribution and locality of these plants, while the molecular means to identify the species. Foundational Biodiversity Information Programme South Africa (Western Cape, Kwa-Zulu Natal)
2020-06-29T12:06:44.347+02:00 dataset Pietersen G (2020): FBIP: Taxonomy and Candidatus Liberibacter status of South African Buchu plants. v1.1. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=taxonomy&v=1.1 http://ipt.sanbi.org.za/iptsanbi/resource?id=taxonomy/v1.1.xml